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Phosphoinositides, specially PI(3,4,5)P3, and PI(4,5)P2, are recognized by SHIP2 an associate of the inositol polyphosphate 5-phosphatase family. Therefore, SHIP2 controls both PI(3,4,5)P3 and PI(4,5)P2 levels in undamaged cells. Although the SHIP2 favorite substrate is PI(3,4,5)P3, PI(4,5)P2 may also be dephosphorylated to PI4P. In various glioblastoma cell lines and primary cultures, SHIP2 discoloration in the plasma membrane partly contrasts with PI(4,5)P2 immunoreactivity. Through depletion of SHIP2 in a glioblastoma cell line 1321 N1 cells, we reveal that cell migration is inhibited by SHIP2. Thus, our results reveal a novel role of SHIP2 at the constraint of PI(4,5)P2, PI4P and cell migration in PTEN-deficient glioblastoma N1 cells. Regulation of PI(4,5)P2 and PI4P articles by SHIP2 controls N1 cell migration throughout the company of focal adhesions.

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